Protein - the most abundant class of all biological molecules
See Muscle Evolution Magazine article: Part I |
Part II
The group of highly complex organic compounds found in all living cells and comprising the most abundant class of all
biological molecules. Protein comprises approximately 50% of cellular dry weight. Hundreds of protein molecules have been
isolated in pure, homogeneous form; many have been crystallized. All contain carbon, hydrogen, and oxygen, and nearly all
contain sulfur as well. Some proteins also incorporate phosphorous, iron, zinc, and copper. Proteins are large molecules with
high molecular weights (from about 10,000 for small ones [of 50-100 amino acids] to more than 1,000,000 for certain forms);
they are composed of varying amounts of the same 20 amino acids, which in the intact protein are united through covalent
chemical linkages called peptide bonds. The amino acids, linked together, form linear unbranched polymeric structures called
polypeptide chains; such chains may contain hundreds of amino-acid residues; these are arranged in specific order for a given
species of protein.
Types of Proteins
A protein molecule that consists of but a single polypeptide chain is said to be monomeric; proteins made up of more than one
polypeptide chain, as many of the large ones are, are called oligomeric. Based upon chemical composition, proteins are divided
into two major classes: simple proteins, which are composed of only amino acids, and conjugated proteins, which are composed of
amino acids and additional organic and inorganic groupings, certain of which are called prosthetic groups. Conjugated proteins
include glycoproteins, which contain carbohydrates; lipoproteins, which contain lipids; and nucleoproteins, which contain
nucleic acids.
Classified by biological function, proteins include the enzymes, which are responsible for catalyzing the thousands of chemical
reactions of the living cell; keratin, elastin, and collagen, which are important types of structural, or support, proteins;
hemoglobin and other gas transport proteins; ovalbumin, casein, and other nutrient molecules; antibodies, which are molecules
of the immune system (see immunity); protein hormones, which regulate metabolism; and proteins that perform mechanical work,
such as actin and myosin, the contractile muscle proteins.
Protein Structure
Every protein molecule has a characteristic three-dimensional shape, or conformation. Fibrous proteins, such as collagen and
keratin, consist of polypeptide chains arranged in roughly parallel fashion along a single linear axis, thus forming tough,
usually water-insoluble, fibers or sheets. Globular proteins, e.g., many of the known enzymes, show a tightly folded structural
geometry approximating the shape of an ellipsoid or sphere.
Because the physiological activity of most proteins is closely linked to their three-dimensional architecture, specific terms
are used to refer to different aspects of protein structure. The term primary structure denotes the precise linear sequence of
amino acids that constitutes the polypeptide chain of the protein molecule. Automated techniques for amino-acid sequencing have
made possible the determination of the primary structure of hundreds of proteins.
The physical interaction of sequential amino-acid subunits results in a so-called secondary structure, which often can either be
a twisting of the polypeptide chain approximating a linear helix (alpha-configuration), or a zigzag pattern (ß -configuration). Most
globular proteins also undergo extensive folding of the chain into a complex three-dimensional geometry designated as tertiary
structure. Many globular protein molecules are easily crystallized and have been examined by X-ray diffraction, a technique that
allows the visualization of the precise three-dimensional positioning of atoms in relation to each other in a crystal.
The tertiary structure of several protein molecules has been determined from X-ray diffraction analysis. Two or more polypeptide
chains that behave in many ways as a single structural and functional entity are said to exhibit quaternary structure.
The separate chains are not linked through covalent chemical bonds but by weak forces of association.
The precise three-dimensional structure of a protein molecule is referred to as its native state and appears, in almost all
cases, to be required for proper biological function (especially for the enzymes). If the tertiary or quaternary structure of a
protein is altered, e.g., by such physical factors as extremes of temperature, changes in pH, or variations in salt
concentration, the protein is said to be denatured; it usually exhibits reduction or loss of biological activity.
Protein Synthesis
The cell's ability to synthesize protein is, in essence, the expression of its genetic makeup. Protein synthesis is a
sequence of chemical reactions that occur in four distinct stages, i.e., activation of
the amino acids that ultimately
will be joined together by peptide bonds; initiation of the polypeptide chain at a cell organelle known as the ribosome;
elongation of the polypeptide by stepwise addition of single amino acids to the chain; and termination of amino-acid additions
and release of the completed protein from the ribosome. The information for the synthesis of specific amino-acid sequences is
carried by a nucleic acid molecule called messenger RNA. Proteins are needed in the diet mainly for their
amino acids, which the body uses to build new proteins (see
nutrition).
The mechanism of action of many widely used antibiotics, such as streptomycin, chloramphenicol, and tetracycline, can be
understood in terms of their ability to interfere with some stage of protein synthesis in bacteria.
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